LASE INNOVATION INC.

DNA-optical barcoding technology for spatiotemporal single-cell analysis

LASE INNOVATION INC. operates in national human genome research institute based in Waltham, MA, founded in 2018.

Profile

Abstract
Combining live imaging with single-cell sequencing is a powerful approach for gaining comprehensive
understanding of individual cell behaviors within complex biological systems. However, existing
approaches are limited in throughput, rely on computational data alignments that are prone to bias, or
require analysis of isolated cells. The goal of this Fast-Track SBIR application is to develop a novel cellular
barcoding technology that will enable researchers to conduct both standard live-cell imaging and high-
throughput single-cell sequencing, and then align the two sets of data unambiguously according to the cell
barcodes. The proposed approach uses dual-barcoded laser particles (LPs) with unique pairs of optical
emission spectra and oligonucleotide sequences. These barcodes can be identified either optically during
imaging or by single-cell sequencing. Built on promising preliminary data, Phase I of this project aims to
demonstrate the feasibility of dual-barcoded LPs on a small scale. Upon success of Phase I, Phase II will
streamline and scale up the fabrication of the LPs and test and validate the technology towards
commercialization. The optical barcode readout requires a high-resolution spectrometer and a pump laser.
In Phase II, add-on hardware modules and cell-tracking software will be developed and tested to be
compatible with existing commercial microscopes, m

Within the national human genome research institute space, the company focuses on its core offering rather than a broad product line.

Its base of operations is Waltham, MA.

It is recorded at the SBIR/STTR stage.

The company reports a headcount in the 1-10 range.

It has disclosed roughly $9K in funding.

Website: laseinnovation.com